AHP1388 recognises human CD156c, otherwise known as ADAM10. CD156c is an 84.1 kDa single-pass type I cell membrane protein, which is localized to the plasma membrane but predominantly expressed by the Golgi apparatus.
CD156c is an endopeptidase which has a role in metalloprotease activity and cell-cell matrix interaction. It is responsible for the proteolytic release of several other cell-surface proteins, including TNFa, heparin binding epidermal growth-like factor, ephrin-A2 and CD171. CD156c is also involved in the constitutive and regulated a-secretase cleavage of amyloid precursor protein (APP) and contributes to the normal cleavage of the cellular prion protein.
CD156c is broadly expressed in range of cells and tissues including leucocytes, tumour cells, articular chondrocyes, and in tissue of the spleen, lymph node, thymus, bone marrow, brain and cartilage.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Yes
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunofluorescence
1/250 - 1/1000
Immunohistology - Frozen
Immunohistology - Paraffin(1)
1.0 - 5.0ug/ml
Immunoprecipitation
Western Blotting
1/500 - 1/2000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1388 detects a band of approximately 70kDa (reduced) or 90kDa (non-reduced) in Jurkat cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human CD156c was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by DEAE column chromatography.
京公网安备11010802025653