AHP1385 recognises human Apoptosis Antagonizing Transcription Factor (AATF), also known as Che-1, a ubiquitously expressed nuclear protein.
Research indicates that AATF functions as a general inhibitor of the histone deacetylase HDAC1. It has been also demonstrated that AATF activates transcription factor E2F and that it is involved in the activation of anti-apoptotic pathways.
AATF has been shown to protect neural cells against oxidative damage suggesting it may have therapeutic uses in Alzheimer's Disease.
This antibody has been reported to work in western blotting.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Paraffin(1)
2.5ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human AATF were raised by repeated immunisation of rabbits with highly purified antigen. Ig fraction prepared from whole serum by ammonium sulphate precipitation.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
