AHP1382 recognises the human Fanconi Anemia Group A (FANCA), which is a 163kDa member of the Fanconi anemia complementation group (FANC).
The members of the Fanconi anemia complementation group do not share sequence similarity but are related by their assembly into a common nuclear protein complex. A previously defined member, FANCH, is now known to be identical to FANCA.
FANCA is involved in DNA repair and maybe involved in maintainance of normal chromosome stability and inter-strand DNA cross link repair.
Mutations in the FANCA gene are a common cause of Fanconi anemia (FA), a genetically heterogeneous recessive disorder characterized by progressive pancytopenia, susceptibly to malignancies, cytogenetic instability, hypersensitivity to DNA damaging agents and defective DNA repair.
Species Cross-Reactivity
Target Species
Cross Reactivity
Chimpanzee
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/15000 - 1/60000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
2.5ug/ml
Immunoprecipitation
Western Blotting
1/500 - 1/3000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1382 detects a band of approximately 163kDa in Hela whole cell lysates. An additional band at 130kDa may also be observed.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human FANCA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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