AHP1364 detects a specific epitope within the carboxy-terminal of RING finger protein 25 (RNF25, also named AO7). RNF25 is expressed in a variety of tissues, and has been shown to act as an E3 ligase interacting with many proteins which may be potential ubiquitin ligase substrates.
RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-?B by interacting with p65 upon stimulation with TNF.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/10000 - 1/40000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
1ug
Western Blotting
1/200 - 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1364 detects a band of approximately 54kDa.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to RNF25 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
