AHP1347 detects E2F1 phosphorylated at serine 364 (pSer364). E2F1 is a transcription activator that binds DNA cooperatively with the differentiation regulated transcription factor proteins (DP). Together they bind to the promoter region of a number of genes whose products are involved in cell cycle regulation or DNA replication. E2F1 also binds to the retinoblastoma protein (RB1), which functions in the control of cell-cycle progression from G1 to S phase.
E2F1 can also mediate p53-dependent and independent apoptosis, and phosphorylation of E2F1 at serine 364 results in its stabilisation and apoptosis induction.
E2F1 is a target of the transforming proteins of small DNA tumour viruses and increased expression of this protein has been reported in a variety of cancers.
Species Cross-Reactivity
Target Species
Cross Reactivity
Chimpanzee
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/10000 - 1/60000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
2 - 20ug/ml
Immunoprecipitation
Western Blotting(1)
1/150 - 1/1000
(1)
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1347 detects a band of approximately 47kDa.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to E2F1 (pSer364) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from serum by affinity chromatography.
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