AHP1307 specifically recognises the 55kDa Tryptophan Hydroxylase 2 (TPH2) protein, when phosphorylated at Ser19.
Tryptophan Hydroxylase (TPH) is a rate-limiting enzyme which catalyses the 5-hydroxylation of tryptophan, to form the amino acid 5-hydroxytryptophan (5-HTP), in the first stage of the biosynthesis of the neurotransmitter serotonin (5-hydroxytryptamine/5HT). Mammalian TPH is encoded by two genes: TPH1 expressed in peripheral tissues e.g. skin and gut, and TPH2 primarily expressed in the brain. Studies have demonstrated the regulation/enhancement of TPH activity through phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaMKII), the latter of which phosphorylates a Ser19 site within the regulatory domain of TPH2.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Human
Yes
Mouse
Expected from Sequence
Zebrafish
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting(1)
1/1000
(1)
For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1307 detects a band of approximately 55kDa on lysates from human dorsal Raphe nucleus.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to rat tryptophan hydroxylase 2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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