AHP1303 specifically recognises the 180kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor, when phosphorylated at Ser1480.
Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+blockade and high Ca2+permeability. Studies have shown that the phosphorylation of the Ser1480 site within the C-terminal PDZ ligand of the NR2B subunit of NMDAR, by casein kinase II (CK2), results in a decrease in neuron NR2B surface expression, seeChung, H.J.et al.for details.
The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer’s disease (Popke, E.J.) and also epilepsy and ischemic neuronal cell death.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Chicken
Expected from Sequence
Dog
Expected from Sequence
Human
Expected from Sequence
Mouse
Expected from Sequence
Primate
Expected from Sequence
Zebrafish
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting(1)
1/1000
(1)
For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1303 detects a band of approximately 180kDa in rat hippocampal cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to rat NMDA receptor NR2B subunit were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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