AHP1293 detects the Class II transactivator (CIITA), a predominantly nuclear protein which acts as a positive regulator of class II major histocompatibility complex (MHC-II) gene transcription. MHC-II have important roles in antigen presentation, T-cell selection and activation, and their expression must be tightly regulated to ensure a vigorous response to foreign pathogens whilst minimising damage to host tissues.
CIITA regulates basal and interferon induced MHC-II transcription through interactions with multiple proteins including transcription factors, chromatin modifying enzymes and components of the basal transcription machinery. CIITA regulates MHC-II expression principally in immune cells, but can also affect their expression in endothelial and fibroblast cells.
Mutations in this gene have been associated with bare lymphocyte syndrome type II, which leads to an extreme susceptibility to viral, bacterial and fungal infections. Silencing of CIITA through epigenetic mechanisms also has a major impact on MHC-II expression in tumour cells and can facilitate immune escape.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human CIITA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
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