AHP1292 detects beta-galactosidase, an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides.
Beta-galactosidase is a product of theLacZoperon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/400 - 1/1600
Flow Cytometry
Functional Assays
Immunohistology - Frozen
1/50 - 1/300
Immunohistology - Paraffin
1/50 - 1/300
Western Blotting
1/200 - 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1292 detects a band of approximately 117kDa usingE. colibeta-galactosidase.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to beta-galactosidase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography.
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