AHP1285B is specific for human IL-31 (interleukin-31), a 141 amino acid T-cell derived cytokine and member of the IL-6 cytokine family, which shares homology with oncostatin M, cardiotrophin-1 and LIF (leukaemia inhibitory factor).
IL-31 is preferentially produced by type 2 helper T cells (Th2) and signals through binding to the IL-31 receptor consisting of IL-31 receptor A (IL-31RA/GPL) and oncostatin M receptor B, activators of STAT1, STAT3 and STAT5. IL-31 is an important mediator of T cell mediated immune response and is particularly linked with diseases of the skin, including dermatitis and pruritic atopic skin inflammation, and is also implicated in bronchial inflammation.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human IL-31 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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