AHP1283B is specific for human IL-17F (interleukin-17F), a disulphide-linked homodimer consisting of two 133 amino acid chains, which belongs to the IL-17 gene family and shares the highest sequence homology with IL-17A.
IL-17F is expressed by activated CD4+ T-cells and monocytes, and acts as a stimulator of cytokine production, including granulocyte colony-stimulating factor (GM-CSF), IL-6 and IL-8. IL-7F also stimulates the production of IL-2, MCP-1 (monocyte chemotactic protein-1) and TGFB (Transforming growth factor beta) by endothelial cells, and inhibits endothelial cell angiogenesis.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human IL-17F were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
AHP1283B may be used in a direct ELISA system or as a detection antibody in a sandwich ELISA for human IL-17F in combination withAHP1283as a capture reagent.
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