AHP1257 recognises 5-hydroxymethyluridine, which is created by the oxidation of thymine by ionising radiation or reactive oxygen species. This oxidation causes changes in gene expression, mutations and chromosomal instability all of which play a role in cancer, autoimmune disease and aging. 5-hydroxymethyluridine is a potential biomarker for female breast, colon and rectal cancers, being present at elevated levels, possibly due to impaired DNA repair mechanisms.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/25000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to 5-hydroxymethyluridine were raised by repeated immunisations of goats with highly purified antigen.
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