AHP1161 detects an epitope within the extracellular domain of human SLC5A11, a 75kD Na+/myo-inositol cotransporter also known as SMIT2. SLC5A11 can also cotransport D-glucose and D-xylose, and themyo-inositol (MI) epimer D-chiro-inositol.
MI is the most biologically abundant cyclic polyol, which function as precursors to molecules involved in cell signalling, cell adhesion and vesicular trafficking. MI also controls intracellular osmolarity by serving as a compatible osmolyte, which allows renal cells to function under hypertonic conditions.
SLC5A11 is mainly expressed in the kidney, and particularly in the kidney cortex, where it is reported to be responsible for the apical transport of MI into the proximal tubule. It is also highly expressed in the heart, skeletal muscle, liver and placenta.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
5 - 15 ug/ml
Immunoprecipitation
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to SLC5A11 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.