AHP1139 detects an epitope within the N-terminal (NT) region of the CD98 light chain (CD98LC), also known as the 4F2 light chain, or LAT-1. CD98LC is a 40kD multi-pass membrane protein which forms a heterodimer with the CD98 heavy chain to allow transport of large branched chain, neutral amino acids into the cell. This transport system leads to the uptake of seven amino acids, including isoleucine, phenylalanine, and methionine.
The expression levels of CD98LC are upregulated in a wide spectrum of primary human cancers. Changes in amino acid transporter expression are thought to be an early change in oncogenesis, as they impart growth and survival advantages.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
15 - 25ug/ml
Immunoprecipitation
Western Blotting
1 - 10ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1139 detects a band of approximately 50-55kDa in human liver cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to CD98 light chain were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
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