AHP1103 detects the orphan receptor TR3, a 64kD NR4 Nerve Growth Factor-Like receptor, which belongs to the nuclear hormone receptor family. TR3 acts as a nuclear transcription factor, and can bind to DNA as a monomer, homodimer, or heterodimer with retinoid X receptors. TR3 is unique among nuclear receptors in that it is also an immediate-early gene induced by a diversity of extracellular stimuli, resulting in survival, differentiation or apoptosis.
In cancer TR3 functions as an oncogenic survival factor, but can be a potent inducer of apoptosis when certain death stimuli induce its migration from the nucleus to mitochondria.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
10 - 30ug/ml
Immunoprecipitation
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to TR3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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