AHP1102 recognises an epitope within the C-terminal (CT) region of human phosphatidylinositol transfer protein (PITP) alpha, a cytosolic protein with a molecular weight of 32kD. PITP alpha binds and transfers single phosphatidylinositol or phosphatidycholine molecules between membranes. Accumulating evidence has also suggested that PITP proteins play essential functions in choline-phospholipid metabolism, inositol lipid signalling and vesicular membrane traffic.
PITP alpha is expressed in a wide range of tissues, and is found in both unicellular and multicellular eukaryotes but not bacteria.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Rabbit
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/4000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1 - 3ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1102 detects a band of approximately 32kDa in 293 and human liver lysates. A minimum incubation time of 1 hour is recommended with this antibody.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human PITP alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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