AHP1088 detects an epitope within the C-terminal (CT) region of ErbB3 isoform 1. ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases, and has a molecular weight of 150kD. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain, and can therefore only convey the signal through heterodimerisation with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of cellular pathways, thus leading to proliferation or differentiation.
Amplification of this gene and/or over expression of its protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. In breast cancer it has been shown to dimerize with ErbB2 to drive tumour cell proliferation.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Mouse
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.3ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1088 detects a band of approximately 150kDa in Jurkat cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human ErbB3 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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