AHP1074GA is specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.
AHP1074GA has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.
In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/9000 - 1/450000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/2000 - 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to peptide DYKDDDDK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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