AHP1072 recognises an epitope within the N terminus of androgen receptor (AR), a 95kD ligand-activated transcription factor that belongs to a large family of nuclear receptor proteins. Unbound AR resides predominately in the cytoplasm as a heteromeric complex with hsp90 and other chaperone proteins. These chaperone proteins maintain AR in a form that is receptive to ligand binding.
Dihydrotestosterone (DHT) binds with highest affinity to AR and together with testosterone promotes AR transcriptional activity thereby ensuring the development and maintenance of male reproductive functions. AR homodimers recruit a variety of factors including coactivators and mediator proteins whose enzymatic activities promote chromatin remodeling and transcriptional regulation of target genes leading to cell differentiation, survival, and proliferation.
Mutated AR is strongly implicated in development of prostate adenocarcinoma and metastases development. AR mutations can also cause spinal bulbar muscular atrophy (Kennedy disease) and partial/ complete androgen insensitivity.
Species Cross-Reactivity
Target Species
Cross Reactivity
Chimpanzee
Expected from Sequence
Mouse
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/64000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.3 - 2.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1072 detects a band of approximately 95kDa in human brain and heart cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Androgen receptor were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
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