AHP1065 detects an epitope within the C-terminal of urokinase, a potent 50kD serine protease which is also known as urokinase plasminogen activator (uPA). uPA cleaves and activates plasminogen to form plasmin and is involved in extracellular cellular matrix degradation and cell signalling. Recent studies have implicated uPA and its receptor in the pathogenesis of inflammation and immunity.
A specific polymorphism in the gene encoding uPA may be associated with late-onset Alzheimer’s disease. The levels of uPA, uPAR and its soluble fragments have also been found to be increased in tissues, plasma and other body fluids of cancer patients and to be markers of cancer development and metastasis.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/8000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.3 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1065 detects a band of approximately 48kDa in KEH 293 cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Urokinase were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
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