AHP1063 detects an epitope within the N-terminal (NT) region of formiminotransferase cyclodeaminase (FTCD), also known as 58K Golgi protein. FTCD is a homo-octameric bifunctional enzyme which plays important roles in coupling histidine catabolism with folate metabolism, and integrating the Golgi complex with the vimentin intermediate filament cytoskeleton.
FTCD is present in all mammalian cells either free in the cytosol or tightly bound to the Golgi complex, and is most abundant in the liver. FTCD is linked to two human diseases, autoimmune hepatitis and glutamate formiminotransferase deficiency.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/64000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.03 - 0.5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1063 detects a band of approximately 55-60kDa in human liver lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to FTCD were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
