AHP1061 detects an epitope within the C-terminal (CT) of cofilin 2, an 18kD protein which is involved in the regulation of actin assembly in developing myotubes through the reversible control of actin polymerization and depolymerization. Cofilin 2 is the major component of intranuclear and cytoplasmic actin rods.
Cofilin 2 is expressed in developing skeletal muscle at early foetal stages through to a young postnatal stage. During postnatal development, cofilin 1 expression in muscle is down-regulated and cofilin 2 becomes the only isoform in mature skeletal muscle. The onset of cofilin 2 expression is coupled with terminal differentiation of muscle cells. It is suggested that cofilin 2 could be associated with actin filaments in muscle cells more efficiently than cofilin 1.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Mouse
Expected from Sequence
Pig
Expected from Sequence
Rat
Expected from Sequence
Sheep
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1 - 128,000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.01 - 0.3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1061 detects a band of approximately 18kDa in human duodenum, heart and muscle lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Cofilin 2 were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
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