AHP1020 recognises human Toll-like receptor 5 (TLR5), a 97.6kDa member of the evolutionarily conserved toll-like receptor family (TLRs), expressed at high levels in the liver and detectable in small amounts in most other tissues.
TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. TLR5 acts via a cytokine signaling pathway to induce the activation of NF-kappaB and in turn tumour necrosis factor alpha and has been shown to recognise flagellin from both Gram-positive and Gram-negative bacteria.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin(1)
Immunoprecipitation
Western Blotting
1.0 - 2.0ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1020 detects a band of approximately 97.6kDa in rat brain cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human TLR5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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