AHP1016 recognises murine Noxa, an 11kDa member of the BH3-only proapoptotic protein family. Noxa is localised to the mitochondria and is a transcriptional target of p73, p53 and BH3-only proteins. It is thought to induce apoptosis when activated, lowering the activity of anti-apoptotic Bcl-2-like proteins. It can be upregulated by mitogenic activation, independently of p53. In humans, along with Mcl-1 it is thought to form a selective pathway that restrains lymphocyte proliferation.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin(1)
Immunoprecipitation
Western Blotting
0.5 - 1.0ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1016 detects a band of approximately 11kDa in human stomach cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to NOXA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.