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RABBIT ANTI TSC1

  • 产品编号:AHP1001T      品牌:AbD Serotec       原厂货号:AHP1001T
  • 产品分类:抗体 > 一抗 > 蛋白特异性一抗
  • 应用分类:
 
包装: 50 μg
运保温度: +4oC or -20oC
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描述:

Specificity

AHP1001 recognises an epitope within the internal region of the 130kDa tumour suppressor protein, Tumour sclerosis complex 1 (TSC1). Mutation within the TSC1 gene is responsible for Tuberous sclerosis (TSC), an autosomal dominant genetic disorder associated with the development of benign tumors (hamartomas), a condition also induced by mutation of the Tumour sclerosis complex 2 (TSC2) gene.

Many proteins have been shown to play a role in the regulation of the serine/threonine kinase known as target of rapamycin (TOR), a central component in a complex signaling pathway which controls cell proliferation and cell cycle progression. Negative regulation of TOR activity occurs following the over-expression of the TSC1 and TSC2 gene products, hamartin and tuberin. These act by suppressing the phosphorylation of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) and ribosomal protein S6 kinase (S6K), downstream targets of TOR involved in mRNA translation. Furthermore the TOR activator protein Rheb has been identified as a target of TSC1-TSC2 complexes.

AHP1001 has been reported as suitable for use in IHC on mouse EL4 cell cytospins at 2ug/ml.

Species Cross-Reactivity

Target Species Cross Reactivity
Mouse Yes
Rat Yes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

 

Application Name Yes No Not Determined Suggested Dilution
ELISA        
Flow Cytometry        
Immunohistology - Frozen        
Immunohistology - Paraffin        
Immunoprecipitation        
Western Blotting       1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting

AHP1001 detects a band of approximately 130kDa in mouse EL4 cell lysates.

Storage

Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Antiserum Preparation

Antisera to human TSC1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

GO Terms

UniProt

Health And Safety Information

Material Safety Datasheet Documentation #10040 available at: 
http://www.abdserotec.com/uploads/MSDS/10040.pdf

Regulatory

For research purposes only

 


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