Recombinant fragment of human FAK expressed in E. coli.
Conjugate
Unconjugated
Form
Liquid
Purification
purified
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
1-3 µg/10^6 cells
Immunocytochemistry (ICC)
1-2 ug/ml
Immunofluorescence (IF)
1-2 ug/ml
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:100
Western Blot (WB)
1-2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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