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注意事项:
Thermo-Start DNA Polymerase requires a 15 minute activation step at 95°C.
For maximum sample purity, total RNA should be extracted using a spin column method. The Thermo Scientific Verso cDNA Synthesis Kit should be used in the upstream reverse transcription (RT) step for improved cDNA yield.
Note that ROX dye is light sensitive; exposure should be minimized
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