Solaris qPCR Gene Expression ROX Master Mix

• 产品编号：AB-4351/A      品牌：ABgene       原厂货号：AB-4351/A
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > qPCR
• 应用分类：

描述：

Thermo Scientific Solaris qPCR Master Mix was developed in conjunction with Solaris qPCR Gene Expression Assays for quantification of DNA and cDNA. Optimal qPCR results using probe detection chemistries can be achieved through this system of qPCR products. These optimized master mixes offer a unique feature – they’re blue. Working with blue reagents allows you to see your reagent as you pipette, track your progress across multiple wells, and visually assess your set-up. This visual confirmation further enhances the repeatability of your data.

Highlights

• Increased confidence – the master mix is colored blue, which serves as a visual aid to pipetting, helping to eliminate manual errors that often contribute to variance in qPCR results
• Excellent performance – delivers optimal RT-qPCR assay repeatability, sensitivity and specificity when used in conjunction with Solaris Assays
• Convenient – ready-to-use 2X master mix minimizes setup time

Applications

The Solaris qPCR Master Mix, in combination with Solaris Gene Expression Assays, enables analysis of gene expression in human and mouse.

Includes

With the exception of probe, primers and template the Solaris qPCR Gene Expression Master Mix contains all the components required for reliable, sensitive and specific qPCR detection. These components include:

• Thermo-Start DNA Polymerase – a chemically modified hot-start version of ThermoPrime DNA Polymerase, which prevents non-specific amplification during the reaction set-up.
• Proprietary reaction buffer – a highly optimized buffering system that delivers improved assay performance when used in conjunction with Solaris primer/probe assays. The Solaris Master Mix also contains an inert blue dye to significantly increase the contrast between the reagent and plastic, making verification of master mix dispensing quick, easy and foolproof.
• dNTP – dTTP completely replaces dUTP in the Solaris qPCR Gene Expression Master Mix for maximum amplification efficiency.
• ROX – there are 3 different versions of the Solaris master mix; Solaris ROX master mix, Solaris Low ROX Master Mix and Solaris Master Mix with a separate vial of ROX, allowing the end-user to adjust the concentration of ROX in their master mix or exclude it. ROX is a passive reference dye that enables normalization of data on ROX-dependent qPCR platforms.

原厂资料：

注意事项：

Thermo-Start DNA Polymerase requires a 15 minute activation step at 95°C.

For maximum sample purity, total RNA should be extracted using a spin column method. The Thermo Scientific Verso cDNA Synthesis Kit should be used in the upstream reverse transcription (RT) step for improved cDNA yield.

Note that ROX dye is light sensitive; exposure should be minimized.