The Thermo Scientific Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.
Highlights
Highly sensitive with a broad dynamic range: can reverse transcribe RNA from an input range of 1 pg to 1 μg
Efficient RT reaction allows for 75% shorter protocol times (down to 30 minutes)
Unique priming strategy for increased efficiency and broad template flexibility
High affinity RT enzyme effectively transcribes through difficult RNA sequences
Wide working temperature range (42 to 57°C) for success with GC-rich and other difficult templates
Included RT Enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
RNase Inhibitor included
Can be paired with any PCR Kit for complete 2-Step (q)RT-PCR
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