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ABsolute QPCR SYBR Green Fluorescein Mix

 
包装: 200 x 25 μl rxns
运保温度: -20℃
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描述:

Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or a separate ROX vial. A version with no reference dye is also available as well as a master mix optimized for capillary instruments.

 

Highlights

  • High Sensitivity & Specificity – The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
  • Optimised buffer containing SYBR Green I
  • Reproducibility – All master mixes undergo extensive QC to ensure batch to batch consistency
  • Broad dynamic range

 

Applications

  • Gene expression analysis
  • siRNA validation
  • Genotyping

 

Absolute qPCR SYBR Green Fluorescein Mix contains 2X Master Mix supplemented with fluorescein.


注意事项:

Note that SYBR Green and ROX dyes are light sensitive; exposure should be minimized.


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