Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or a separate ROX vial. A version with no reference dye is also available as well as a master mix optimized for capillary instruments.
Highlights
High Sensitivity & Specificity – The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
Optimised buffer containing SYBR Green I
Reproducibility – All master mixes undergo extensive QC to ensure batch to batch consistency
Broad dynamic range
Applications
Gene expression analysis
siRNA validation
Genotyping
Absolute qPCR SYBR Green Mix (no ROX) contains 2X Master Mix and no passive reference dye.
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注意事项:
Note that SYBR Green and ROX dyes are light sensitive; exposure should be minimized.