ReadiLink™ KLH Conjugation Kit

产品编号：AAT-5502 品牌：AAT 原厂货号：5502
产品分类：生化和细胞分析 > 标签、标记试剂盒 > 生物偶联物 > 其他偶联物
应用分类：

包装：

1 kit

运保温度:

低于2-4℃温度下冷藏保存

到货周期：

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其它组分：

mcKLH【包装：2 × 2mg，，运保温度：Store at < -20°C】

Conjugation buffer (pH 4.7)【包装：20 mL，，运保温度：Store at 4 °C】

EDC (1-ethy 1-3-[dimethylaminopropyl] carbodiimide hydrochloride)【包装：2 × 10 mg，，运保温度：Store at 4 °C】

Purification buffer (pH 7.2)【包装：2 x 10 mL，，运保温度：Store at < -20°C】

Spin Desalting Columns (7K MWCO)【包装：2 × 2 mL，，运保温度：Store at 4 °C *Do not freeze*】

描述：

Keyhole Limpet Hemocyanin (KLH) is one of the most commonly used carriers in the conjugation of peptides for antibody production. Mariculture keyhole limpet hemocyanin (mcKLH) is a hemocyanin from the Concholepas concholepas mollusk with immunogenic properties similar to KLH but is more stable and efficient as a carrier protein for the production of antibodies to haptens and peptides. It contains numerous sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers. mcKLH is currently the industry standard for antibody production against a hapten or peptide. This ReadiLink™ KLH Conjugation kit is primarily optimized for the simple preparation of hapten-carrier conjugates for immunization and antibody production. The ReadiLink™ KLH Conjugation kit is one-step conjugation of a hapten to a carrier protein using the carboxyl-reactive carbodiimide as the crosslinker. The resulting conjugate is used for eliciting an immune response and antibody production against the hapten. The carboxyl-reactive carbodiimide reacts with exposed carboxyl and amino groups on peptides and proteins to form stable bonds. These kits contain mcKLH formulated in buffers compatible with the carboxyl-reactive carbodiimide reactions and desalt spin columns, which offer exceptional protein recovery by simple centrifugation step.