Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This kit uses a proprietary water-soluble tetrazolium dye that produces a water-soluble formazan dye upon cellular reduction in the presence of an electron carrier (such as PMS). The tetrazolium salt is reduced by cellular dehydrogenases to an orange formazan product that is soluble in culture medium. The amount of formazan produced is directly proportional to the number of living cells. Our kit provides a more robust protocol than other commercial tetrazolium-based cytotoxicity assay kits (such as MTT or XTT kits). No pre-mixing of components is required for our kit since the tetrazolium dye solution is added directly to the cells. In addition, our kit has higher sensitivity than the assays using the other tetrazolium salts (such as MTT and XTT). It can be readily adapted for a wide variety of instrument platforms. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized assay protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
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