Our Cell Navigator™ fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label mitochondria of live cells in orange fluorescence of large Stokes Shift at Ex/Em = 405/550 nm. The kit uses a proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency. The mitochondrial stain used in the kit is quite photostable, making the fluorescence quite robust. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
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