Our Cell Explorer™ Live Cell Tracking Kit uses a proprietary orange fluorescent dye that gets enhanced fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The fluorescent dye used in the kit has the spectral properties of Ex/Em = ~530/550 nm. The tracking dye has good photostability with robust imaging performance. The kit is particularly suitable for multicolor flow cytometric analysis of cells. It can also be used with a fluorescence microscope equipped with a TRITC filter set. This kit provides an effective tool of labeling cells for flow cytometric and fluorescence microscopic investigations of cellular functions. The effective labeling of cells offers a powerful method for studying cellular events in a spatial and temporal context. This kit is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. The kit provides all the essential components with an optimized cell-labeling protocol.
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