It is a cell-permeable version of calcein blue. Upon getting into live cells the weakly fluorescent calcein blue AM is hydrolyzed into calcein blue that has the excitation/emission maxima similar to those of DAPI, Hoechst and AMCA. This exceptional spectral separation from the typical green and red fluorophores (such as FITC, TMR and Texas Red) provides additional options for multiplexing experiments. Because calcein blue AM is intrinsically fluorescent, a proper filter set and additional wash step may be necessary to minimize background fluorescence. Our calcium assay buffers may be used to effectively wash extracellular calcein blue.
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