Ethidium bromide is an intercalating agent commonly used as a nucleic acid stain in molecular biology laboratories for techniques such as agarose gel electrophoresis. It has almost 20-fold fluorescence enhancement upon binding to DNA. Single-stranded RNA can also be detected, since it usually folds back onto itself and thus provides local base pairing for the dye to intercalate. Ethidium bromide is a very strong mutagen, and may possibly be a carcinogen or teratogen.
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