Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

• 产品编号：AAT-13500      品牌：AAT       原厂货号：13500
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 蛋白酶/肽酶检测
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描述：

Our Amplite. Universal Fluorimetric Protease Activity Assay Kit is an ideal choice to perform routine assays for the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. The kit uses a fluorescent casein conjugate which is proven to be a generic substrate for a broad spectrum of proteases (e.g. trypsin, chymotrypsin, thermolysin, proteinase K, protease XIV, and elastase). In the intact substrate, casein is heavily labeled with a green fluorescent dye, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. The assay can be performed in a convenient 96-well or 384-well microtiter plate format and readily adapted to automation. Its signal can be easily read with a fluorescence microplate reader at Ex/Em = 490/525 nm using FITC filter set.

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