AAT Bioquest's iFluor™ dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor™ 750 dyes have fluorescence excitation and emission maxima close to 750 nm and 780 nm respectively with good photostability. Our in-house comparable studies indicated that our iFluor™ 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen respectively). iFluor™ 780 conjugates have been widely used in fluorescence animal imaging applications. The iFluor™ 750 SE dye used in the kit is reasonably stable and shows good reactivity and selectivity with proteins. ReadiLink™ iFluor™ 750 Protein Labeling Kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa). ReadiLink™ labeling kits require minimal hands-on time without a column purification steps. The kits provide all the essential components with only step of mixing and a simple spinning down. AAT Bioquest offers three different sizes to meet both exploration and research needs. This iFluor™ 750 labeling kit is optimized for labeling ~100 µg antibody.
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