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ReadiLink™ iFluor™ 647 Protein Labeling Kit *Microscale Optimized for Labeling 100 μg An

 
包装: 1 kit
运保温度: Freeze (<-15 °C) Avoid light
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其它组分:

iFluor™ 647 SE【包装:1 vial,,运保温度:-20 °C】
Reaction Buffer【包装:1 vial (200 μL),,运保温度:Do not freeze】
DMSO【包装:1 vial (100 μL),,运保温度:-20 °C】
Spin Column【包装:2 columns,,运保温度:Do not freeze】
Washing Tube (2 mL)【包装:2 tubes,,运保温度:Do not freeze】
Collecting Tube (1.5 mL)【包装:2 tubes,,运保温度:Do not freeze】


描述:

AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor™ 555 dyes have fluorescence excitation and emission maxima of ~550 nm and ~570 nm respectively. iFluor™ 647 family has the spectral properties essentially identical to those of Cy5® (Cy5® is the trademark of GE Healthcare). Compared to Cy5 probes iFluor™ 647 family has much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a superior alternative to Cy5®. iFluor™ 647 family has become an excellent replacement to Cy5 and Alexa Fluor® 647 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor™ 647 SE used in the ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. This kit has all the essential components for labeling ~100 ug antibody. iFluor™ 647 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 647 SE.


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