Our Amplite. Fluorimetric ELISA Assay Kit contains all the essential components including our fluorogenic Amplite. Red HRP substrate for ELISA detection. The kit provides an optimized assay protocol. It can detect as little as 0.4 ng/well of a monoclonal antibody. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = ~540/590 nm or an absorbance microplate reader at ~576 nm. It has been used for the assays in which goat anti-mouse IgG is served as a secondary detection agent.