This Amplite™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit uses our unique ROS Green™ hydrogen peroxide sensor to quantify hydrogen peroxide in live cells. ROS Green™ is cell-permeable, and generates the green fluorescence when it reacts with hydrogen peroxide. The kit is an optimized “mix and read” assay format that is compatible with HTS liquid handling instruments. The Amplite™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit provides a sensitive, one-step fluorometric assay to detect as little as 0.3 nanomoles of H2O2 in a 100 μL assay volume (3 μM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 490/520 nm for H2O2 detection in solution or a fluorescence microscopy for live cell H2O2 imaging.