This Amplite™ Hydrogen Peroxide Assay Kit uses our unique Amplite™ IR peroxidase substrate to quantify hydrogen peroxide in solutions and cell extracts. Amplite™ IR generates the fluorescence that is pH-independent from pH 4 to 10. Thus it is superior alternative to ADHP (Amplex Red™) for the detections that require low pH where ADHP (Amplex Red™) has reduced fluorescence. In addition, Amplite™ IR generates a product that has maximum absorption of 647 nm with maximum emission at 670 nm. This near infrared absorption and fluorescence minimize the assay background that is often caused by the autofluorescence of biological samples that rarely absorb light beyond 600 nm. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is compatible with HTS liquid handling instruments.
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