Our Amplite™ Fluorimetric Acetylholine Assay Kit provides one of the most sensitive methods for quantifying acetylcholine. The kit uses Amplite™ Red to quantify acetylcholine through the choline oxidase-mediated enzyme coupling reactions. The fluorescence intensity of Amplite™ Red is proportional to acetylcholine formation. The kit is an optimized “mix and read” assay. It provides an ultrasensitive one-step fluorimetric assay to detect as little as 0.01 nanomoles ACh in a 100 μL assay volume (0.1 μM). Its signal can be easily read with a fluorescence microplate reader at Ex/Em = ~540/590 nm or an absorbance microplate reader at ~576 nm.
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