Our Amplite™ Colorimetric Acetylcholinesterase Assay Kit provides a convenient method for the detection of AChE activity. It uses DTNB to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE in blood, in cell extracts, and in other solutions. The absorption intensity of DTNB adduct is used to measure the amount of thiocholine formed, which is proportional to the AChE activity. The kit provides a colorimetric one-step assay to detect as little as 0.1 mU AChE in a 100 μL assay volume (1 mU/mL) as shown in Figure 1. Its signal can be easily read by an absorbance microplate reader at ~410 nm. The kit is robust and can be used for continuously monitoring AChE activities.
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