Amplite™ Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

• 产品编号：AAT-11302      品牌：AAT       原厂货号：11302
• 产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 其它酶检测
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描述：

The Amplite. Glutamate Oxidase Assay Kit provides a quick and an ultrasensitive method for the measurement of glutamate oxidase in solution and in cell lysates. In the assay, L-glutamic acid is oxidized to α-ketoglutarate, NH3 and H2O2 by glutamate oxidase. The kit uses our Amplite. Red substrate which can react with H2O2 when catalyzed by horseradish peroxidase (HRP) to generate the highly fluorescent product. The signal can be read with either a fluorescence microplate reader at Ex/Em = 530-570 nm/590 -600 nm (optimal Ex/Em = 540 nm/590 nm) or an absorbance microplate reader at 576刡5 nm. With the Amplite. Glutamate Oxidase Assay kit, we have detected as little as 40 U/mL glutamate oxidase in a 100 L reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

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