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Amplite™ Fluorimetric Myelopeoxidase Assay Kit *Red Fluorescence*

 
包装: 1 kit
运保温度: Keep at -20°C and protect from light
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其它组分:

Amplite™ Red (light sensitive)【包装:1 vial】
Assay Buffer【包装:20 mL】
H2O2【包装:100μL (3%)】
Myeloperoxidase Standard【包装:1 vial (10 mU, lyophilized)】
DMSO【包装:1vial (200 μL)】


描述:

Our Amplite. Fluorimetric Myeloperoxidase Assay Kit provides a quick and sensitive method for the measurement of myeloperoxidase in solution and in cell lysates. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The kit uses our Amplite. Red substrate which enables a dual recordable mode. The signal can be easily read by either a fluorescence microplate reader at Ex/Em = 530-570 nm/590-600 nm (optimal Ex/Em = 540 nm/590 nm) or an absorbance microplate reader at 576刡5 nm. With the Amplite. Myeloperoxidase Assay Kit, we have detected as little as 0.1 mU/mL myeloperoxidase in a 100 冚L reaction volume.


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