Our Amplite. Fluorimetric Myeloperoxidase Assay Kit provides a quick and sensitive method for the measurement of myeloperoxidase in solution and in cell lysates. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The kit uses our Amplite. Red substrate which enables a dual recordable mode. The signal can be easily read by either a fluorescence microplate reader at Ex/Em = 530-570 nm/590-600 nm (optimal Ex/Em = 540 nm/590 nm) or an absorbance microplate reader at 576刡5 nm. With the Amplite. Myeloperoxidase Assay Kit, we have detected as little as 0.1 mU/mL myeloperoxidase in a 100 冚L reaction volume.