Our Amplite™ IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2. It can be used to detect both H2O2 and peroxidase. Amplite™ IR generates a substance that has maximum absorption of 647 nm with maximum emission at 670 nm. This near infrared absorption and fluorescence minimize the assay background that is often caused by the autoabsorption and/or autofluorescence of biological samples that rarely absorb light beyond 600 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of Amplite™ IR is minimal. Compared to ADHP (also called Amplex Red™ in literature) Amplite™ IR generates the fluorescence that is pH-independent from pH 4 to 10. Thus it is superior alternative to ADHP (Amplex Red™) for the detections that require low pH where ADHP (Amplex Red™) has reduced fluorescence. We have used Amplite™ IR to detect HRP in quite a few immunoassays. Amplite™ IR can also be used to detect trace amount of H2O2. Because H2O2 is produced in many enzymatic redox reactions, Amplite™ IR can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids etc.
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