AAM55B is specific for mouse Interleukin-15 (IL-15), an immunomodulatory cytokine that stimulates the proliferation of T lymphocytes and acts as a maturation factor for NK cells.
The stimulation of cell growth by IL-15, occurs through the binding of IL-15 to the IL-2 receptor and, although IL-15 and IL-2 exhibit similar biological properties, there is no sequence homology between them. Increased IL-15 expression is linked with inflammatory bowel disease, rheumatoid arthritis and HIV affiliated diseases.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.15 - 0.30ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to mouse IL-15 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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