AAM34B is specific for mouse Interleukin-13 (IL-13), a 12.3kDa immunoregulatory cytokine produced primarily by activated T helper type 2 (Th2) cells and also by mast cells and NK cells.
The biological properties of IL-13 include stimulation of B cell proliferation and antibody production and studies suggest that IL-13 plays an important role in the induction of the pathophysiological features of allergic asthma, independently of Immunoglobulin E and eosinophils, seeWills-Karp, M.et al.for details.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Western Blotting
AAM34B may used in western blotting under either reducing or non-reducing conditions withPMP46as the positive control.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to mouse IL-13 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
